1. Molecular Cloning: A Laboratory Manual, Sambrook and D.W. Russell, ed., Cold Spring Harbor Laboratory Press

2. S. B. Primrose and R. M. Twyman. Principles of Gene Manipulationand Genomics, 7th Edn, Blackwell Publishing

3. J. J. Greene and V. B. Rao. Recombinant DNA Principles and Methodologies. CRC Press

Students either studying or completed, BSc (research), MSc, B.Tech, B.Pharam., BVSc., MBBS, B.Pharm., MS (Biotech), or Equivalent.

Basic knowledge in the Life Science

Growth and maintenance of recombinant bacterial strains. Transformation and transfection methods. Vectors used in molecular cloning and expression of genes. DNA, RNA, and protein isolation, purification, and fractionation methods. Enzymes used in genetic engineering. Radioactive and non-radioactive labeling of nucleic acids and proteins and their detection. Nucleic acid hybridization methods. Gene and cDNA cloning methods. Construction of genomic DNA and cDNA libraries. Nucleic acid sequencing methods, including Next-Generation Sequencing. Methods for protein analysis, protein-nucleic acid, and protein-protein interactions. Site-specific mutagenesis. Polymerase chain reaction, Real-time Quantitative PCR., and applications. Antisense technology and RNA silencing techniques. Recombinant protein production in bacteria, yeast, and mammalian cells, Genome editing approaches such as Cas9/CRISPR technology. Exome Sequencing- Ch1P Sequencing. Generation of Lentiviral, retroviral and Adenoviral vectors, and Gene therapy, Genetic Engineering of mammalian stem cells, Generation of induced pluripotent stem (iPS) cells, Somatic cell nuclear transfer, Generation of transgenic and mutant Caenorhabditis elegans –Generation of knock-out mice (isolation and culture of embryonic stem (ES) cells, Gene is targeting construct design, Transfection, Homologous recombination in ES Cells, Positive and negative selection; Breeding of germ-line chimeras Cre/lox and Flp/FRT system for inducible transgenic mice – Chemically inducible transgene expression systems. Use of transgenic technology in modeling human diseases, including cardiovascular disease, diabetes, obesity, cancer, atherosclerosis, neurodegenerative diseases, muscle degeneration, and aging